Mapping of antigenic sites on the nucleocapsid protein of the severe acute respiratory syndrome coronavirus.
Identifieur interne : 005251 ( Main/Exploration ); précédent : 005250; suivant : 005252Mapping of antigenic sites on the nucleocapsid protein of the severe acute respiratory syndrome coronavirus.
Auteurs : Yuxian He [États-Unis] ; Yusen Zhou ; Hao Wu ; Zhihua Kou ; Shuwen Liu ; Shibo JiangSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps antiviraux (sang), Anticorps monoclonaux (immunologie), Anticorps monoclonaux (sang), Biologie informatique (), Cartographie épitopique, Données de séquences moléculaires, Humains, Hybridomes, Immunisation, Protéines nucléocapside (), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Séquence d'acides aminés, Virus du SRAS (immunologie), Épitopes immunodominants (immunologie).
- MESH :
- génétique : Protéines nucléocapside.
- immunologie : Anticorps antiviraux, Anticorps monoclonaux, Protéines nucléocapside, Syndrome respiratoire aigu sévère, Virus du SRAS, Épitopes immunodominants.
- sang : Anticorps antiviraux, Anticorps monoclonaux.
- Animaux, Biologie informatique, Cartographie épitopique, Données de séquences moléculaires, Humains, Hybridomes, Immunisation, Protéines nucléocapside, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Monoclonal (blood), Antibodies, Monoclonal (immunology), Antibodies, Viral (blood), Antibodies, Viral (immunology), Computational Biology (methods), Epitope Mapping, Humans, Hybridomas, Immunization, Immunodominant Epitopes (immunology), Mice, Mice, Inbred BALB C, Molecular Sequence Data, Nucleocapsid Proteins (chemistry), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control).
- MESH :
- chemical , blood : Antibodies, Monoclonal, Antibodies, Viral.
- chemical , chemistry : Nucleocapsid Proteins.
- chemical , genetics : Nucleocapsid Proteins.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Viral, Immunodominant Epitopes, Nucleocapsid Proteins.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- methods : Computational Biology.
- prevention & control : Severe Acute Respiratory Syndrome.
- Amino Acid Sequence, Animals, Epitope Mapping, Humans, Hybridomas, Immunization, Mice, Mice, Inbred BALB C, Molecular Sequence Data.
Abstract
Antigenic sites on the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) were mapped by Pepscan analysis with overlapping peptides that span the N protein sequence. Two major immunodominant epitopes located in the C-terminal region (amino acids [aa] 362 to 412) and middle region (aa 153 to 178) reacted with more than 75% of sera from SARS patients. Several minor immunodominant epitopes were reactive with about 50% of the SARS sera. Antisera from mice immunized with inactivated SARS-CoV recognized the two major immunodominant epitopes and one antigenic site located adjacent to the N-terminal region (aa 76 to 101), which did not react with the sera from SARS patients. Several monoclonal antibodies against SARS-CoV bound to the N- or C-terminal antigenic sites. These results suggest that the above antigenic sites on the N protein are important in eliciting humoral immune response against SARS-CoV in humans and animals and can be used as antigens for developing diagnostic tests.
DOI: 10.1128/JCM.42.11.5309-5314.2004
PubMed: 15528730
Affiliations:
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Kimball Research Institute, New York Blood Center, New York, NY 10021, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Lindsley F. 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xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (immunologie)</term><term>Anticorps antiviraux (sang)</term><term>Anticorps monoclonaux (immunologie)</term><term>Anticorps monoclonaux (sang)</term><term>Biologie informatique ()</term><term>Cartographie épitopique</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Hybridomes</term><term>Immunisation</term><term>Protéines nucléocapside ()</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Séquence d'acides aminés</term><term>Virus du SRAS (immunologie)</term><term>Épitopes immunodominants (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Monoclonal</term><term>Antibodies, Viral</term></keywords><keywords 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sévère</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Antigenic sites on the nucleocapsid (N) protein of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) were mapped by Pepscan analysis with overlapping peptides that span the N protein sequence. Two major immunodominant epitopes located in the C-terminal region (amino acids [aa] 362 to 412) and middle region (aa 153 to 178) reacted with more than 75% of sera from SARS patients. Several minor immunodominant epitopes were reactive with about 50% of the SARS sera. Antisera from mice immunized with inactivated SARS-CoV recognized the two major immunodominant epitopes and one antigenic site located adjacent to the N-terminal region (aa 76 to 101), which did not react with the sera from SARS patients. Several monoclonal antibodies against SARS-CoV bound to the N- or C-terminal antigenic sites. These results suggest that the above antigenic sites on the N protein are important in eliciting humoral immune response against SARS-CoV in humans and animals and can be used as antigens for developing diagnostic tests.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region></list><tree><noCountry><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><name sortKey="Kou, Zhihua" sort="Kou, Zhihua" uniqKey="Kou Z" first="Zhihua" last="Kou">Zhihua Kou</name><name sortKey="Liu, Shuwen" sort="Liu, Shuwen" uniqKey="Liu S" first="Shuwen" last="Liu">Shuwen Liu</name><name sortKey="Wu, Hao" sort="Wu, Hao" uniqKey="Wu H" first="Hao" last="Wu">Hao Wu</name><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="He, Yuxian" sort="He, Yuxian" uniqKey="He Y" first="Yuxian" last="He">Yuxian He</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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